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Urotensin II promotes the proliferation and secretion of vascular endothelial growth factor in rat dermal papilla cells by activating the Wnt-β-catenin signaling pathway

Authors

Congjuan Liao
People’s Hospital of Longgang District & The Second Hospital of the Chinese University of Hong Kong, Shenzhen, China.
Zhen Huang
Shenzhen Hospital of Beijing University of Chinese Medicine, Shenzhen, China.
Liuting Chen
Shenzhen Hospital of Beijing University of Chinese Medicine, Shenzhen, China.
Xiaorong Fan
People’s Hospital of Longgang District & The Second Hospital of the Chinese University of Hong Kong, Shenzhen, China.
Jun Peng
People’s Hospital of Longgang District & The Second Hospital of the Chinese University of Hong Kong, Shenzhen, China.
Xiaoqing Tan
People’s Hospital of Longgang District & The Second Hospital of the Chinese University of Hong Kong, Shenzhen, China.
Jianshe Yang
yangjs@impcas.ac.cn
https://orcid.org/0000-0001-7069-6072
Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China.
Xusheng Zhang
People’s Hospital of Longgang District & The Second Hospital of the Chinese University of Hong Kong, Shenzhen, China.

Introduction. Urotensin II (U II) is a kind of active peptide with a variety of biological effects, such as promoting cell proliferation and endocrine effects. The aim of this study is to investigate the effect of urotensin II on the proliferation and secretion of vascular endothelial growth factor (VEGF) in cultured rat dermal papilla cells (DPCs), and to explore its molecular mechanism. Materials and Methods. We used the DPCs isolated from the thoracic aortas of Wistar-Kyoto rats to run the CCK8 and ELISA assay, RC-PCR and Western blotting techniques to identify the effect of Urotensin II on the proliferation and secretion of VEGF in DPCs, data were analyzed by one-way ANOVA or t-test. Results. U II can increase the mRNA expression of proliferation markers Ki67 and PCNA. In addition, the Wnt/β-catenin pathway was activated by U II, but Wnt inhibitor DKK1 reversed the effect of U II. Conclusions. U II promoted the proliferation and secretion of VEGF in rat DPCs through activation of the Wnt-β-catenin signaling pathway.

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Supporting Agencies

This work was supported by the Special Fund for Economic and Scientific Development in Longgang District, Shenzhen City, Guangdong Province (No. LGWJ2022-32).

How to Cite

Liao, C., Huang, Z., Chen, L., Fan, X., Peng, J., Tan, X., Yang, J., & Zhang, X. (2023). Urotensin II promotes the proliferation and secretion of vascular endothelial growth factor in rat dermal papilla cells by activating the Wnt-β-catenin signaling pathway. Italian Journal of Medicine, 17(1). https://doi.org/10.4081/itjm.2023.1607
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